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APS reverses ROS generation and mitochondrial membrane disruption in ATO-induced BMSCs. Cells were treated with 0.5 μmol/L ATO, or 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. <t>(A)</t> <t>DCFH-DA</t> staining (Scale bar: 100 μm, ×10). (B) Quantification of ROS generation, n = 3. (C) JC-1 staining (Scale bar: 20 μm, ×40). (D) Quantification of JC-1 staining, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.
Fluorescent Probes Dcfh Da, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent probes dcfh da/product/MedChemExpress
Average 97 stars, based on 1 article reviews
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APS reverses ROS generation and mitochondrial membrane disruption in ATO-induced BMSCs. Cells were treated with 0.5 μmol/L ATO, or 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. <t>(A)</t> <t>DCFH-DA</t> staining (Scale bar: 100 μm, ×10). (B) Quantification of ROS generation, n = 3. (C) JC-1 staining (Scale bar: 20 μm, ×40). (D) Quantification of JC-1 staining, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.
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APS reverses ROS generation and mitochondrial membrane disruption in ATO-induced BMSCs. Cells were treated with 0.5 μmol/L ATO, or 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. <t>(A)</t> <t>DCFH-DA</t> staining (Scale bar: 100 μm, ×10). (B) Quantification of ROS generation, n = 3. (C) JC-1 staining (Scale bar: 20 μm, ×40). (D) Quantification of JC-1 staining, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.
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APS reverses ROS generation and mitochondrial membrane disruption in ATO-induced BMSCs. Cells were treated with 0.5 μmol/L ATO, or 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. <t>(A)</t> <t>DCFH-DA</t> staining (Scale bar: 100 μm, ×10). (B) Quantification of ROS generation, n = 3. (C) JC-1 staining (Scale bar: 20 μm, ×40). (D) Quantification of JC-1 staining, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.
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APS reverses ROS generation and mitochondrial membrane disruption in ATO-induced BMSCs. Cells were treated with 0.5 μmol/L ATO, or 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. (A) DCFH-DA staining (Scale bar: 100 μm, ×10). (B) Quantification of ROS generation, n = 3. (C) JC-1 staining (Scale bar: 20 μm, ×40). (D) Quantification of JC-1 staining, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.

Journal: Chinese Herbal Medicines

Article Title: Astragalus polysaccharides inhibit arsenic trioxide-induced BMSCs damage through inhibition of Jnk and p38 signaling pathways

doi: 10.1016/j.chmed.2025.03.007

Figure Lengend Snippet: APS reverses ROS generation and mitochondrial membrane disruption in ATO-induced BMSCs. Cells were treated with 0.5 μmol/L ATO, or 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. (A) DCFH-DA staining (Scale bar: 100 μm, ×10). (B) Quantification of ROS generation, n = 3. (C) JC-1 staining (Scale bar: 20 μm, ×40). (D) Quantification of JC-1 staining, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.

Article Snippet: Oxidation-sensitive fluorescent probes (DCFH-DA) (MedChemExpress, New Jersey, USA, Cat. No. HY-D0940) were used to detect the intracellular ROS.

Techniques: Membrane, Disruption, Staining, Two Tailed Test, Control